In vitro protein refolding by chromatographic procedures.
نویسندگان
چکیده
In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.
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ورودعنوان ژورنال:
- Protein expression and purification
دوره 33 1 شماره
صفحات -
تاریخ انتشار 2004